10 Augustus 2025
| 12:15 |
PEC Zwolle 
1 - 0
FC Twente '65 
|
17 Augustus 2025
| 14:30 |
FC Twente '65
0 - 2
PSV 
|
24 Augustus 2025
| 12:15 |
SC Heerenveen 
1 - 2
FC Twente '65
|
30 Augustus 2025
| 21:00 |
SBV Excelsior 
1 - 0
FC Twente '65
|
13 September 2025
| 21:00 |
FC Twente '65
2 - 2
NAC Breda 
|
19 September 2025
| 20:00 |
Sparta Rotterdam 
1 - 5
FC Twente '65
|
26 September 2025
| 20:00 |
FC Twente '65
3 - 2
Fortuna Sittard 
|
05 Oktober 2025
| 12:15 |
FC Twente '65
2 - 1
Heracles Almelo 
|
18 Oktober 2025
| 16:30 |
NEC 
3 - 3
FC Twente '65
|
26 Oktober 2025
| 12:15 |
FC Twente '65
2 - 3
AFC Ajax 
|
30 Oktober 2025
| 21:00 |
RKSV Rohda Raalte 
1 - 4
FC Twente '65
|
02 November 2025
| 14:30 |
FC Groningen 
1 - 1
FC Twente '65
|
07 November 2025
| 20:00 |
FC Twente '65
0 - 0
Telstar 1963 
|
22 November 2025
| 18:45 |
FC Volendam 
1 - 1
FC Twente '65
|
30 November 2025
| 16:45 |
FC Twente '65
1 - 0
AZ 
|
07 December 2025
| 12:15 |
FC Utrecht 
1 - 1
FC Twente '65
|